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Rat Glucagon Like Peptide 1 (GLP-1) ELISA Kit

Catalog Number: orb1806835

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1806835
CategoryAssays and Kits
DescriptionRat Glucagon Like Peptide 1 (GLP-1) ELISA Kit
TargetGLP1
ReactivityRat
UniProt IDP06883
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GLP-1. You can calculate the concentration of Rat GLP-1 in the samples by comparing the OD of the samples to the standard curve.
Assay TypeSandwich
Assay Time3.5H
Standard100pg/mL
Range1.56-100pg/mL
Sensitivity0.94 pg/mL
Sample Typesserum, plasma, Tissue homogenate and Other biological samples
Alternative namesGLP1
NoteFor research use only
Rat Glucagon Like Peptide 1 (GLP-1) ELISA Kit

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