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Rat Ghrelin (GHRL) ELISA Kit

Catalog Number: orb1806837

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1806837
CategoryAssays and Kits
DescriptionRat Ghrelin (GHRL) ELISA Kit
ReactivityRat
Range0.16-10ng/mL
Assay Time1.5H
TargetGHRL
Sensitivity0.1ng/mL
Alternative namesGHRL, MTLRP, Ghrelin
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NoteFor research use only
Application notesThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat GHRL. During the reaction, Rat GHRL in the sample or standard competes with a fixed amount of Rat GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Expiration DatePlease enquire.
Rat Ghrelin (GHRL) ELISA Kit

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