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| Catalog Number | orb2667390 |
|---|---|
| Category | Assays and Kits |
| Description | Rat Free Testosterone (F-Testo) ELISA Kit |
| Range | 31.25-2000 pg/mL |
| Assay Time | 2.5h |
| Sensitivity | 16.3 pg/mL |
| Alternative names | F-TESTO; Free Testosterone Read more... |
| Note | For research use only |
| Application notes | standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat F-Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F-Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F-Testo in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, other biological fluids |
| Expiration Date | Please enquire. |
Rat F-TESTO ELISA Kit [orb411339]
Rat
0.3 pg/mL-60 pg/mL
0.15 pg/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T
