Rat Focal Adhesion Kinase (FAK) ELISA Kit

SKU: orb780986

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Focal Adhesion Kinase(FAK).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FAK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FAK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FAK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FAK in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.116 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PTK2 ELISA Kit, FADK ELISA Kit, FAK1 ELISA Kit, Pp125FAK ELISA Kit, PPP1R71 ELISA Kit, Protein Tyrosine Kinase 2 ELISA Kit, Focal adhesion kinase-related nonkinase ELISA Kit, Protein phosphatase 1 regulatory subunit 71 ELISA Kit

Quick Database Links

UniProt

O35346