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Rat Fatty Acid Synthase (FASN) ELISA Kit

Catalog Number: orb779859

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb779859
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Synthase(FASN).
Tested applicationsELISA
ReactivityRat
Range0.32-20 ng/mL
Assay Time3.5h
Concentration20 ng/mL
UniProt IDP12785
Sensitivity0.127 ng/mL
Alternative namesFAS ELISA Kit, OA519 ELISA Kit, SDR27X1 ELISA Kit,
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NoteFor research use only
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FASN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FASN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FASN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FASN in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.
Rat Fatty Acid Synthase (FASN) ELISA Kit

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