Rat Fatty Acid Binding Protein 5, Epidermal (FABP5) ELISA Kit

SKU: orb781546

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 5, Epidermal(FABP5).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FABP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FABP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FABP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FABP5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.072 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

E-FABP ELISA Kit, EFABP ELISA Kit, PA-FABP ELISA Kit, PAFABP ELISA Kit, Psoriasis-Associated ELISA Kit, Epidermal-type fatty acid-binding protein ELISA Kit, Psoriasis-associated fatty acid-binding protein homolog ELISA Kit

Quick Database Links

UniProt

P55053