Rat Fatty Acid Binding Protein 4, Adipocyte (FABP4) ELISA Kit

SKU: orb780045

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 4, Adipocyte(FABP4).

Research Area

Cardiovascular Research, Gastroenterology & Hepatology, Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FABP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FABP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FABP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FABP4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.65 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

A-FABP ELISA Kit, AFABP ELISA Kit, AP2 ELISA Kit, Adipocyte Protein 2 ELISA Kit, Adipocyte-type fatty acid-binding protein ELISA Kit

Quick Database Links

UniProt

P70623