Rat Eukaryotic Initiation Factor 4E (eIF4E) ELISA Kit

SKU: orb782346

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to eIF4E(eukaryotic initiation factor 4E).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 93.75-6000 pg/mL
• Sensitivity: 26.14 pg/mL
• Concentration: 6000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CBP ELISA Kit, eIF 4E ELISA Kit, eIF 4F 25 kDa subunit ELISA Kit, EIF4E ELISA Kit, EIF4E1 ELISA Kit, EIF4EL1 ELISA Kit, EIF4F ELISA Kit

Quick Database Links

UniProt

P63074