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| Catalog Number | orb2322418 |
|---|---|
| Category | Assays and Kits |
| Description | Rat Enkephalin (ENK) ELISA Kit |
| Reactivity | Rat |
| Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ENK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ENK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ENK in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 3.53 pg/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | ENK |
| Note | For research use only |
Rat ENK ELISA Kit [orb411045]
Rat
0.625 ng/mL-40 ng/mL
0.156 ng/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T- Item 1 of 1
