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| Catalog Number | orb780448 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3B(ELA3B). |
| Reactivity | Rat |
| Concentration | 10 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ELA3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ELA3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ELA3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ELA3B in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.054 ng/mL |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | CELA3B ELISA Kit, Chymotrypsin Like Elastase Famil Read more... |
| Note | For research use only |
