Rat E-Cadherin (E-Cad) ELISA Kit

• Catalog Number: orb2667145
• Category: Assays and Kits
• Description: Rat E-Cadherin (E-Cad) ELISA Kit
• UniProt ID: Q9R0T4
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.13 ng/mL
• Sample Types: serum, plasma, urine, tissue homogenates and other biological fluids
• Alternative names: CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO;
• Note: For research use only