Rat Dual Serine/Threonine and Tyrosine Protein Kinase (DSTYK) ELISA Kit

SKU: orb781046

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Serine/Threonine And Tyrosine Protein Kinase(DSTYK).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DSTYK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DSTYK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DSTYK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DSTYK in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.115 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

RIPK5 ELISA Kit, Dusty PK ELISA Kit, RIP5 ELISA Kit, SgK496 ELISA Kit, Receptor Interacting Protein Kinase 5 ELISA Kit, RIP-homologous kinase ELISA Kit, Sugen kinase 496 ELISA Kit, Receptor-interacting serine/threonine-protein kinase 5 ELISA Kit

Quick Database Links

UniProt

Q6XUX2