Rat Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit

SKU: orb782697

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DLD(Dihydrolipoyl Dehydrogenase).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DLD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DLD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DLD in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.064 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

DLD ELISA Kit, Diaphorase ELISA Kit, DLD ELISA Kit, DLDD ELISA Kit, DLDH ELISA Kit, GCSL ELISA Kit, LAD ELISA Kit, Lipoamide Dehydrogenase ELISA Kit, PHE3 ELISA Kit, 2-oxo-glutaRate complex ELISA Kit, branched chain keto acid dehydrogenase complex ELISA Kit, branched chain keto acid dehydrogenase complex ELISA Kit, dihydrolipoamide dehydrogenase ELISA Kit, E3 component of pyruvate dehydrogenase complex ELISA Kit, dihydrolipoamide dehydrogenasemitochondrial ELISA Kit, DLDH ELISA Kit, E3 ELISA Kit, E3 component of pyruvate dehydrogenase complex ELISA Kit, 2-oxo-glutaRate complex ELISA Kit, EC 1.8.1 ELISA Kit, EC 1.8.1.4 ELISA Kit, GCSL ELISA Kit, Glycine cleavage system L protein ELISA Kit, glycine cleavage system protein L ELISA Kit, lipoamide dehydrogenase ELISA Kit, lipoamide reductase ELISA Kit, lipoyl dehydrogenase ELISA Kit

Quick Database Links

UniProt

Q6P6R2