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| Catalog Number | orb775879 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1(CYP2E1). |
| Reactivity | Rat |
| Concentration | 200 ng/mL |
| UniProt ID | P05182 |
| Tested applications | ELISA |
| Application notes | standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYP2E1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYP2E1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYP2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CYP2E1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 3.13-200 ng/mL |
| Sensitivity | 1.41 ng/mL |
| Sample Types | Tissue homogenates, cell lysates and other biological fluids |
| Alternative names | CPE1 ELISA Kit, CYPIIE1 ELISA Kit, P450-J ELISA Ki Read more... |
| Note | For research use only |
Rat CYP2E1 ELISA Kit [orb411226]
Rat
0.156 ng/mL-10 ng/mL
0.039 ng/mL
10 x 96 T, 5 x 96 T, 48 T, 96 T, 24 T
