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| Catalog Number | orb2667310 |
|---|---|
| Category | Assays and Kits |
| Description | Rat Cystatin C (Cys-C) ELISA Kit |
| UniProt ID | P14841 |
| Application notes | standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 1.57-100 ng/mL |
| Sensitivity | 0.51 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin |
| Note | For research use only |
Rat Cystatin C ELISA Kit [orb410748]
Rat
0.312 ng/ml-20 ng/ml
0.078 ng/ml
24 T, 48 T, 10 x 96 T, 96 T, 5 x 96 T- Item 1 of 1
