Rat Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit

• Catalog Number: orb780018
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 1A(CDKN1A).
• Tested applications: ELISA
• Reactivity: Rat
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• Concentration: 10 ng/mL
• Sensitivity: 0.058 ng/mL
• Alternative names: WAF1 ELISA Kit, p21 ELISA Kit, CIP1 ELISA Kit, p21
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CDKN1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CDKN1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CDKN1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CDKN1A in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.

References

Reham L Younis 1, Rehab M El-Gohary 2, Asmaa A Ghalwash 2, Islam Ibrahim Hegab 1 3, Maram M Ghabrial 4, Azza M Aboshanady 4, Raghad A Mostafa 5, Alaa H Abd El-Azeem 6, Eman E Farghal 5, Asmaa A E Belal 7, Haidy Khattab Luteolin Mitigates D-Galactose-Induced Brain Ageing in Rats: SIRT1-Mediated Neuroprotection Neurochem Res, (2024)

PubMed: 38987448

Applications

ELISA

Reactivity

Rat