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Rat Cyclic ADP Ribose Hydrolase (cADPRH) ELISA Kit

Catalog Number: orb777676

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb777676
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclic ADP Ribose Hydrolase(cADPRH).
Tested applicationsELISA
ReactivityRat
Range3.13-200 ng/mL
Assay Time3.5h
Concentration200 ng/mL
UniProt IDQ64244
Sensitivity1.24 ng/mL
Alternative namesCD38 ELISA Kit, ADPRC 1 ELISA Kit, c-ADPRH ELISA K
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NoteFor research use only
Application notesstandard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat cADPRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat cADPRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat cADPRH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat cADPRH in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates and other biological fluids.
Expiration DatePlease enquire.
Rat Cyclic ADP Ribose Hydrolase (cADPRH) ELISA Kit