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Rat Coupling Factor 6 (CF6) ELISA Kit

Rat Coupling Factor 6 (CF6) ELISA Kit

Catalog Number: orb2667464

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667464
CategoryAssays and Kits
DescriptionRat Coupling Factor 6 (CF6) ELISA Kit
Range0.16-10 ng/mL
Assay Time3.5h
Sensitivity0.064 ng/mL
Alternative namesATP5J; ATP5A; ATP5; ATPM; ATP Synthase,H+ Transpor
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NoteFor research use only
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CF6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CF6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CF6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CF6 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates and other biological fluids.
Expiration DatePlease enquire.