Rat Corticotropin Releasing Hormone (CRH) ELISA Kit

• Catalog Number: orb2667854
• Category: Assays and Kits
• Description: Rat Corticotropin Releasing Hormone (CRH) ELISA Kit
• UniProt ID: P01143
• Application notes: standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CRH in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.14 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: CRF; Corticotropin Releasing Factor; Corticoliberi
• Research Area: Cardiovascular Research, Kidney & Urological Resea
• Note: For research use only
• Expiration Date: Please enquire.