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| Catalog Number | orb776745 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 43(CX43). |
| Reactivity | Rat |
| Concentration | 20 ng/mL |
| UniProt ID | P08050 |
| Tested applications | ELISA |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CX43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CX43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CX43 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.121 ng/mL |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Alternative names | ODDD ELISA Kit, GJAL ELISA Kit, GJA1 ELISA Kit, GJ Read more... |
| Note | For research use only |
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