Rat Complement C5 Protein, His Tag

SKU: orb570266

Description

Rat Complement C5, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 1688 (Accession # XP_001079130.2).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 74.1 kDa (β chain) and 114.8 kDa (α chain). The protein migrates as 70 kDa and 110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: Complement C5
• Reactivity: Rat
• Tag: C-10×His
• Molecular Weight: 74.1 kDa (β chain) & 114.8 kDa (α chain)
• Expression Region: Gln 24 - Ala 1688
• Purity: 95%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Rat Complement C5, His Tag (Cat. No. orb570266) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-12.5 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5

UniProt

XP_001079130.2

RefSeq:XP_001079130.2