Rat Coagulation Factor VII (F7) ELISA Kit

SKU: orb2667571

Description

Rat Coagulation Factor VII (F7) ELISA Kit

Research Area

Hematology, Infectious Disease & Virology, Metabolism Research

Images & Validation

• Application Notes:
  standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F7 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Plasma
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 12.4 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa

Quick Database Links

UniProt

Q8K3U6