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| Catalog Number | orb1088147 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase(CERK). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ceramide Kinase(CERK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide Kinase(CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ceramide Kinase(CERK) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Rat |
| Concentration | 200 ng/mL |
| Application notes | standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CERK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CERK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CERK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CERK in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 3.13-200 ng/mL |
| Sensitivity | 1.16 ng/mL |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Alternative names | LK4, hCERK, Lipid kinase 4, Acylsphingosine kinase |
| Note | For research use only |
