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Catalog Number | orb707318 |
---|---|
Category | Proteins |
Description | Recombinant Rat CD86 Protein is produced by mammalian expression system and the target gene encoding Vla29-Lys247 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Rat |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CD86 |
Entrez | 56822 |
UniProt ID | O35531 |
Protein Sequence | VPVKRQAYFN STAYLPCPFT KAQNISPSEL VVFWQDRKKS VLYEHYLGAE KLDNVNAKYL GRTSFDRDNQ ALRLHNVQIK DTGLYDCFIQ QKTPTGSIIL QQWETELSVI ANFSEPEIEE AQNETRNTGI NLTCSSKQGY PKPTKMYFLI TNSTNEYGDN MQISQDNVTK LFSVSISLSL PFPDGVYNMT IVCILETESM NISSKPHNMV FSQPQFDRKH HHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | CD28LG2 protein, T-lymphocyte activation antigen C Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
26.8 kDa (predicted); 40-70 kDa (reducing condition, due to glycosylation) |
> 95%, determined by SDS-PAGE | |
This protein contains the rat CD86 (NP_064466.1) extracellular domain (Met 1-Ile 250) was expressed from HEK293 Cells. |