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| Catalog Number | orb781026 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Acetyltransferase(CRAT). |
| Reactivity | Rat |
| Concentration | 200 ng/mL |
| UniProt ID | Q704S8 |
| Tested applications | ELISA |
| Application notes | standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 3.13-200 ng/mL |
| Sensitivity | 1.27 ng/mL |
| Sample Types | Tissue homogenates, cell lysates and other biological fluids |
| Alternative names | CAT1 ELISA Kit, Carnitine O-Acetyltransferase ELIS Read more... |
| Note | For research use only |
