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Rat Cardiotrophin 1 (CT1) ELISA Kit

Catalog Number: orb775432

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb775432
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cardiotrophin 1(CT1).
Tested applicationsELISA
ReactivityRat
Range15.63-1000 pg/mL
Assay Time3.5h
Concentration1000 pg/mL
UniProt IDQ63086
Sensitivity5.6 pg/mL
Alternative namesCTF1 ELISA Kit
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NoteFor research use only
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CT1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.
Rat Cardiotrophin 1 (CT1) ELISA Kit

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