Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit

SKU: orb775950

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Gamma(CAMK2g).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CAMK2g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CAMK2g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CAMK2g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CAMK2g in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.116 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CAMKII ELISA Kit, CAMK2G ELISA Kit, CAMK ELISA Kit, CAMK-II ELISA Kit, CAMKG ELISA Kit, CaM Kinase ELISA Kit, CaM kinase II subunit gamma ELISA Kit, CaMK-II subunit gamma ELISA Kit

Quick Database Links

UniProt

Q13555