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Rat Calcitonin (CT) ELISA Kit

Catalog Number: orb1817296

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1817296
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CT in the samples is then determined by comparing the OD of the samples to the standard curve.
ReactivityRat
Concentration1000 pg/mL
UniProt IDP01258
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CT in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range15.63-1000 pg/mL
Sensitivity6 pg/mL
Sample Typestissue homogenates, cell lysates and other biological fluids
Alternative namesCALCA; CALC; Calcitonin carboxyl-terminal peptide
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NoteFor research use only
Rat Calcitonin (CT) ELISA Kit

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