Rat BMP8A(Bone Morphogenetic Protein 8A) Microsample ELISA Kit

SKU: orb2997697

Description

The Rat BMP8A(Bone Morphogenetic Protein 8A) Microsample ELISA Kit is designed to quantify BMP8A(Bone Morphogenetic Protein 8A) Microsample in rat serum, plasma and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.107 ng/mL.

Research Area

Stem Cell & Developmental Biology; Musculoskeletal & Connective Tissue Research

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMP8A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMP8A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMP8A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMP8A in the samples is then determined by comparing the OD of the samples to the standard curve.

  Read more...

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.107 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BMP8-A