Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

SKU: orb777177

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-Site APP Cleaving Enzyme 1(bACE1).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat bACE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat bACE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat bACE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat bACE1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma, cerebrospinal fluid and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.7 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ASP2 ELISA Kit, Beta Secretase ELISA Kit, Memapsin 2 ELISA Kit, Aspartyl protease 2 ELISA Kit, Beta-site amyloid precursor protein cleaving enzyme 1 ELISA Kit, Membrane-associated aspartic protease 2 ELISA Kit

Quick Database Links

UniProt

P56819

References

Shams Tabrez et al. Therapeutic potential of SSZ in modulating Alzheimer's disease pathology: A multi- targeted experimental approach researchgate.net, (2026)

PubMed:

Applications

ELISA

Reactivity

Rat