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| Catalog Number | orb2667708 |
|---|---|
| Category | Assays and Kits |
| Description | Rat Beta-2-Microglobulin (BMG/b2-MG) ELISA Kit |
| Range | 0.32-20 µg/mL |
| Assay Time | 3.5h |
| UniProt ID | P07151 |
| Sensitivity | 0.11 µg/mL |
| Alternative names | Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin Read more... |
| Note | For research use only |
| Application notes | standard: 20 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
