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| Catalog Number | orb779541 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein(Bax). |
| Tested applications | ELISA |
| Reactivity | Rat |
| Range | 12.5-800 pg/mL |
| Assay Time | 3.5h |
| Concentration | 800 pg/mL |
| UniProt ID | Q63690 |
| Sensitivity | 4.7 pg/mL |
| Alternative names | BCL2L4 ELISA Kit, Bax Zeta ELISA Kit, Apoptosis re Read more... |
| Note | For research use only |
| Application notes | standard: 800 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Bax. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bax. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Bax, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bax in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
| Expiration Date | Please enquire. |
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