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| Catalog Number | orb779186 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter G2(ABCG2). |
| Reactivity | Rat |
| Concentration | 20 ng/mL |
| UniProt ID | Q80W57 |
| Tested applications | ELISA |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ABCG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ABCG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ABCG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ABCG2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.108 ng/mL |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Alternative names | CDw338 ELISA Kit, CD338 ELISA Kit, ABC-G2 ELISA Ki Read more... |
| Note | For research use only |
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