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Rat Arginine Vasopressin (AVP) ELISA Kit

Catalog Number: orb782678

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb782678
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin (AVP).
ReactivityRat
Concentration20 ng/mL
UniProt IDP01186
Tested applicationsELISA
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AVP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AVP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AVP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AVP in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.116 ng/mL
Sample TypesTissue homogenates and other biological fluids.
Alternative namesARVP ELISA Kit, AVP ELISA Kit, VP ELISA Kit, Argin
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NoteFor research use only
Rat Arginine Vasopressin (AVP) ELISA Kit