Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit

SKU: orb779606

Description

The Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit uses a sandwich enzyme immunoassay to quantify Arachidonate-5-Lipoxygenase (ALOX5) in rat tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.79-50 ng/mL and a sensitivity of 0.28 ng/mL.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALOX5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALOX5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALOX5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALOX5 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.79-50 ng/mL
• Sensitivity: 0.28 ng/mL
• Concentration: 50 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

LOX5 ELISA Kit, 5-LOX ELISA Kit, ALOX-5 ELISA Kit, 5-LO ELISA Kit, 5LPG ELISA Kit, LOG5 ELISA Kit, Lipoxygenase 5 ELISA Kit

Quick Database Links

UniProt

P12527