Rat AP(Aprotinin) ELISA Kit

SKU: orb2999143

Description

Rat AP(Aprotinin) ELISA Kit

Research Area

Cardiovascular Research, Gastroenterology & Hepatology, Molecular Biology, Protein Biochemistry

Images & Validation

• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AP in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Competitive
• Assay Time: 2h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.47 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor