Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

SKU: orb780482

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin II Receptor 1(AGTR1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AGTR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGTR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AGTR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGTR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.052 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

AT1R ELISA Kit, AG2S ELISA Kit, AG2-S ELISA Kit, AGTR1A ELISA Kit, AGTR1B ELISA Kit, AT1 ELISA Kit, AT1B ELISA Kit, AT2R1 ELISA Kit, AT2-R1 ELISA Kit, AT2R1A ELISA Kit, AT2R1B ELISA Kit, HAT1R ELISA Kit, AT1 Receptor ELISA Kit, Angiotensin Receptor 1B ELISA Kit

Quick Database Links

UniProt

P25095