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Catalog Number | orb1807084 |
---|---|
Category | Assays and Kits |
Description | Rat Angiotensin Ⅱ (Ang-Ⅱ) ELISA Kit |
Reactivity | Rat |
Range | 15.63-1000pg/mL |
Assay Time | 1.5H |
Target | AngII |
Sensitivity | 9.38pg/mL |
Alternative names | Angiotensin-2 Read more... |
Note | For research use only |
Application notes | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-Ⅱ. During the reaction, Rat Ang-Ⅱ in the sample or standard competes with a fixed amount of Rat Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
Sample Types | Serum, Plasma, Tissue homogenate and Other biological samples |
Expiration Date | Please enquire. |
Rat | |
15.6 pg/ml-1000 pg/ml. | |
7.8 pg/ml. |