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Rat Angiotensin Ⅱ (Ang-Ⅱ) ELISA Kit

Catalog Number: orb1807084

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1807084
CategoryAssays and Kits
DescriptionRat Angiotensin Ⅱ (Ang-Ⅱ) ELISA Kit
ReactivityRat
Range15.63-1000pg/mL
Assay Time1.5H
TargetAngII
Sensitivity9.38pg/mL
Alternative namesAngiotensin-2
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NoteFor research use only
Application notesThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-Ⅱ. During the reaction, Rat Ang-Ⅱ in the sample or standard competes with a fixed amount of Rat Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Expiration DatePlease enquire.
Rat Angiotensin Ⅱ (Ang-Ⅱ) ELISA Kit

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