Rat Androgen Receptor (AR) ELISA Kit

SKU: orb779867

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Androgen Receptor(AR).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.066 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

AIS ELISA Kit, DHTR ELISA Kit, HUMARA ELISA Kit, KD ELISA Kit, NR3C4 ELISA Kit, SBMA ELISA Kit, SMAX1 ELISA Kit, TFM ELISA Kit, Dihydrotestosterone receptor ELISA Kit, Nuclear Receptor Subfamily 3,Group C,Member 4 ELISA Kit, Testicular Feminization ELISA Kit, Kennedy Disease ELISA Kit

Quick Database Links

UniProt

P15207