Rat Amyloid Beta 1-42 (Aβ1-42) Microsample ELISA Kit

• Catalog Number: orb1806633
• Category: Assays and Kits
• Description: Rat Amyloid Beta 1-42 (Aβ1-42) Microsample ELISA Kit
• Target: Aβ1-42
• Reactivity: Rat
• Application notes: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Aβ1-42. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Rat Aβ1-42. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat Aβ1-42. You can calculate the concentration of Rat Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.
• Assay Time: 3H
• Range: 15.63-1000pg/mL
• Sensitivity: 9.38 pg/mL
• Sample Types: Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL
• Alternative names: Aβ42,Amyloid Beta 42
• Note: For research use only