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Rat Amphiregulin (AREG) ELISA Kit

Catalog Number: orb776333

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776333
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amphiregulin(AREG).
Tested applicationsELISA
ReactivityRat
Range15.63-1000 pg/mL
Assay Time3.5h
Concentration1000 pg/mL
UniProt IDP24338
Sensitivity6.3 pg/mL
Alternative namesAR ELISA Kit, CRDGF ELISA Kit, SDGF ELISA Kit, Col
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NoteFor research use only
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AREG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AREG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AREG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AREG in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
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