Rat Alpha Crosslaps (α-CTx) ELISA Kit

• Catalog Number: orb1946804
• Category: Assays and Kits
• Description: Rat Alpha Crosslaps (α-CTx) ELISA Kit
• Target: α-CTx
• Reactivity: Rat
• Application notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat α-CTx. During the reaction, Rat α-CTx in the sample or standard competes with a fixed amount of Rat α-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat α-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat α-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 6000pg/mL
• Range: 93.75-6000pg/mL
• Sensitivity: 56.25 pg/mL
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Alternative names: a-CTXI, aCTX-I, A-Cr, Type I Collagen C-Telopeptid
• Research Area: Immunology & Inflammation
• Note: For research use only