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Rat Aldosterone Synthase (ALDOS) ELISA Kit

Catalog Number: orb775811

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb775811
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldosterone Synthase(ALDOS).
ReactivityRat
Concentration10 ng/mL
UniProt IDP30099
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALDOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALDOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALDOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALDOS in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.056 ng/mL
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Alternative namesCPN2 ELISA Kit, CYP11B ELISA Kit, CYP11BL ELISA Ki
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NoteFor research use only
Rat Aldosterone Synthase (ALDOS) ELISA Kit