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| Catalog Number | orb2667169 |
|---|---|
| Category | Assays and Kits |
| Description | Rat Afamin (AFM) ELISA Kit |
| Range | 3.13-200 µg/mL |
| Assay Time | 2.5h |
| UniProt ID | P36953 |
| Sensitivity | 0.89 µg/mL |
| Alternative names | ALB2; ALBA; ALF; A-ALB; Alpha-Albumin Read more... |
| Note | For research use only |
| Application notes | standard: 200 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AFM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AFM in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma and other biological fluids. |
| Expiration Date | Please enquire. |
