Rat Afamin (AFM) ELISA Kit

• Catalog Number: orb2667169
• Category: Assays and Kits
• Description: Rat Afamin (AFM) ELISA Kit
• UniProt ID: P36953
• Application notes: standard: 200 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AFM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AFM in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 3.13-200 µg/mL
• Sensitivity: 0.89 µg/mL
• Sample Types: Serum, plasma and other biological fluids.
• Alternative names: ALB2; ALBA; ALF; A-ALB; Alpha-Albumin
• Research Area: Cancer Biology, Gastroenterology & Hepatology, Inf
• Note: For research use only
• Expiration Date: Please enquire.