Rat Adrenomedullin (ADM) ELISA Kit

SKU: orb778739

Description

The Rat Adrenomedullin (ADM) ELISA Kit uses a sandwich enzyme immunoassay to quantify Adrenomedullin (ADM) in rat serum, plasma and other biological fluids. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 5.8 pg/mL.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADM in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.8 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

AM ELISA Kit, PAMP ELISA Kit, Proadrenomedullin N-20 terminal peptide ELISA Kit, ProAM N-terminal 20 peptide ELISA Kit

Quick Database Links

UniProt

P43145