Rat 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

SKU: orb2667361

Description

Rat 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit

Research Area

Cancer Biology, Gastroenterology & Hepatology, Hematology, Infectious Disease & Virology, Metabolism Research

Images & Validation

• Application Notes:
  standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 1.56-100 ng/mL
• Sensitivity: 0.46 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine