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| Catalog Number | orb782160 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 6-keto-PGF1 alpha protein. |
| Tested applications | ELISA |
| Reactivity | Rat |
| Range | 7.8 pg/ml - 500 pg/ml |
| Assay Time | 2.5h |
| Concentration | 500 pg/mL |
| Sensitivity | 1.4 pg/mL |
| Alternative names | 6-keto-PGF1 alpha ELISA Kit, 6ketoPGF1α ELISA Kit Read more... |
| Note | For research use only |
| Application notes | standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 6ketoPGF1α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 6ketoPGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 6ketoPGF1α in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma and other biological fluids. |
| Expiration Date | Please enquire. |
