Rat 5'-Nucleotidase, Cytosolic II (NT5C2) ELISA Kit

SKU: orb781373

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-Nucleotidase, Cytosolic II(NT5C2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT5C2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT5C2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT5C2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT5C2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.128 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NT5B ELISA Kit, PNT5 ELISA Kit, GMP ELISA Kit, cN-II ELISA Kit, NT5CP ELISA Kit, Purine 5' Nucleotidase ELISA Kit, 5'-Nucleotidase(Purine),Cytosolic Type B ELISA Kit, Cytosolic purine 5'-nucleotidase ELISA Kit

Quick Database Links

UniProt

D3ZMY7