RafRBDGST (C-Raf)

SKU: orb533598

Description

Raf is a member of the serine/threonine proteine kinase family involved in regulation of cell growth and differentiation and is the most important effector of Ras. Full-length Raf is composed of three conserved regions responsible for interaction with Ras, for phos-phorylation and for catalytic activity. RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf. The GST-Tag facilitates the protein‘s application in typical GST pull-down assays. The protein is GST-tagged on its N-terminus. There is a 7 AA linker between GST and RBD.

Research Area

Signal Transduction

Key Properties

• Source: E. coli
• Biological Origin: human
• MW: Theoretical MW: 42 kDa
• Purity: > 90% (SDS-PAGE)

Storage & Handling

• Storage: store at -80 °C
• Form/Appearance: liquid (Supplied in PBS, pH 7.4)
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Ras Binding Domain of Raf, C-Raf 1 Kinase, human, recombinant, E. coli