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| Catalog Number | orb1173553 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Carbonyl(PC). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Carbonyl(PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Carbonyl(PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Carbonyl(PC) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Rabbit |
| Concentration | 20 nmol/mL |
| Tested applications | ELISA |
| Application notes | standard: 20 nmol/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit PC in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.32-20 nmol/mL |
| Sensitivity | 0.114 nmol/mL |
| Sample Types | Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Lysate |
| Alternative names | Protein Carbonyl |
| Note | For research use only |
